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fbp1 polyclonal antibody  (Proteintech)


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    Proteintech fbp1 polyclonal antibody
    a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, <t>Fbp1</t> + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.
    Fbp1 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fbp1+polyclonal+antibody/FBP1+Antibody/pmc11484945-210-26-31
    Average 93 stars, based on 46 article reviews
    fbp1 polyclonal antibody - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Single-cell spatiotemporal analysis of the lungs reveals Slamf9 + macrophages involved in viral clearance and inflammation resolution"

    Article Title: Single-cell spatiotemporal analysis of the lungs reveals Slamf9 + macrophages involved in viral clearance and inflammation resolution

    Journal: Cell Discovery

    doi: 10.1038/s41421-024-00734-4

    a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, Fbp1 + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.
    Figure Legend Snippet: a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, Fbp1 + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.

    Techniques Used: Infection, Staining, Control



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    a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, <t>Fbp1</t> + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.
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    a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, <t>Fbp1</t> + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.
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    a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, <t>Fbp1</t> + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.
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    Image Search Results


    a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, Fbp1 + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.

    Journal: Cell Discovery

    Article Title: Single-cell spatiotemporal analysis of the lungs reveals Slamf9 + macrophages involved in viral clearance and inflammation resolution

    doi: 10.1038/s41421-024-00734-4

    Figure Lengend Snippet: a Cell fate inference analysis by CellRank based on monocyte/macrophage scRNA-seq data at d7 and d14. b Boxplot showing the proportion of Trem2 + AMs, Fbp1 + AMs and proliferating Fbp1 + AMs in total Stereo-seq bin80-bins of nine lung slides at each timepoint. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test. c RNA velocity analysis of AM subpopulations. Arrows indicate the potential directions of state transitions. d Boxplot showing the spatial correlation between different cell types and Fbp1 + AMs of nine lung slides at d14. Data are represented as mean ± SEM ( n = 9 slides per timepoint). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Kruskal–Wallis test was performed to calculate the P value across different groups. Pearson correlation was performed to calculate the P value of all spots within each slide. Black dots: P < 0.05; gray dots: P ≥ 0.05. Red dotted line represents the correlation value of 0. e , f Representative images of the lung sections of hACE2 mice before and after SARS-CoV-2 infection detected by immunofluorescent staining. TREM2 and CD68 at d0 and d14 ( e ); FBP1 and CD68 at d0 and d14 ( f ). TREM2 + or FBP1 + macrophages were indicated by arrows. Scale bars, 50 μm. g Boxplot showing the proportion of SLAMF9 + SPP1 + , TREM2 + , FBP1 + , FBP1 + MKI67 + , and MARCO + CD36 + TREM2 – FBP1 – macrophages in CD68 + macrophages in scRNA-seq data of human lung autopsy samples of control ( n = 8) and COVID-19 patients ( n = 5). Center line, median; box bounds, first and third quartiles; whiskers, 1.5 times the interquartile range. Data are represented as mean ± SEM. Two-sided Wilcoxon test.

    Article Snippet: Different primary antibodies were sequentially applied to examine specific cell markers, including anti-CD68 Polyclonal antibody (28058-1-AP, 1:800; Proteintech) and TREM2 Polyclonal antibody (13483-1-AP, 1:200; Proteintech), or FBP1 Polyclonal antibody (12842-1-AP, 1:100, Proteintech), or Osteopontin Polyclonal antibody (22952-1-AP, 1:200; Proteintech) and SLAMF9 polyclonal antibody (LM-2205R, 1:200; LMAI BIO), followed by HRP-conjugated secondary antibody incubation and tyramide signal amplification (TSA).

    Techniques: Infection, Staining, Control